Several theories have been put forward concerning the origin of the
neoplastic epithelium in ameloblas- toma. Because of the doubt on the
possible origin of ameloblastomas from cells with odontogenic
potentiality, the aim of the present was to observe the development and
growth pattern of odontogenic epithelium and tooth germs in tissue
culture. Fourteen molar tooth germs consisting enamel organ and dental
papilla of fourteen days old mouse embryos were cultured in a medium on
millipore filters. THWP 0.45 m porosity and 25 ± 5 m thin. Cultivation
was terminated at 21st day. Explants were embedded in cellulose-paraffin
for histology. After 2 days cultivation, the epithelial outgrowth of
enamel organ was observed, and 2 days later was visible structure
resembled the pattern of the dental lamina often seen in ameloblastomas.
Histological sections of specimens showed that the outer epithelium has
differentiated into a lamellar-like structure and the enamel organ has
similar pattern of the ameloblastoma. Formation of microcysts and
continued formation of epithelial buds was observed. Tissue culture
studies of mouse embryo tooth germs and enamel organ demostrated
epithelial proliferation from the borders of odontogenic epithelium and
the formation of lamellar- like structure observed in ameloblastomas.
This study offers additional experimental evidence that ameloblastomas
orginate from epithelium with odontogenic potentiality.
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